We are currently seeking a motivated and talented scientist to join our team as PhD student in the Molecular Biology Lab led by Dr. Martin Fischer as part of the Computational Biology Group headed by Prof. Dr. Steve Hoffmann.
We study how transcription factors control gene regulatory networks. For example, the transcription factor p53 serves as a central suppressor of tumor progression. It controls cell proliferation and apoptosis by regulating a plethora of target genes. However, it is not clear how p53 regulates many of its target genes, what factors besides p53 itself are necessary for up or downregulation and what their regulation contributes to a normal or cancer cell. Our incomplete picture of the molecular basis of p53-dependent gene regulation remains a critical gap to our overall understanding of tumor suppression.
· An internationally competitive PhD project in a young research team
· Top-notch facilities and experimental support
· Productive collaborations and plentiful opportunities to further develop professional skills within a multi-disciplinary research team at the Leibniz-Institute on Aging
· The PhD position will be integrated in our Leibniz Graduate School on Aging and Age Related Diseases. The contract conditions and the salary will be according to the collective labor agreement for public service employees of the Federal State of Germany (TV-L E13), 50 % during the first two years and 60 % during the last two years.
· The project is interdisciplinary involving molecular biology experiments and computational analyses
· Cell culture work including transfections
· Molecular biology (Cloning, RNA and DNA extraction, real time RT-PCR, Chromatin Immunoprecipitation, DNA affinity purification, SDS PAGE and Western Blot)
· RNA-Seq and ChIP-Seq including data analysis
· Recent degree (M.Sc. or equivalent) in Biochemistry, Biology or a related discipline of the Life Sciences
· Strong background in molecular biology (experience in the methods described above)
· Strong commitment and work ethic
Please acquaint yourself with the LGSA procedures and with the application procedure on our website lgsa.leibniz-fli.de, fill in the application form and send it electronically to the LGSA.
Selected Publications on this topic:
- Census and evaluation of p53 target genes, Oncogene 2017 (Review)
- Cell cycle transcription control: DREAM/MuvB and RB-E2F complexes; Crit. Rev. Biochem. Mol. Biol. 2017 (Review)
- Integration of TP53, DREAM, MMB-FOXM1 and RB-E2F target gene analyses identifies cell cycle gene regulatory networks. Nucl. Acids Res. 2016
- The p53-p21-DREAM-CDE/CHR pathway regulates G2/M cell cycle genes. Nucl. Acids Res. 2016
- The transcription factor p53: Not a repressor, solely an activator. Cell Cycle 2014